Annotation: Gerechtshof Amsterdam 2008-01-15

Gerechtshof Amsterdam3232FDR Procesrecht en mediation -- ou

Provisionele vordering tegenover kort geding

FDR Procesrecht en mediation -- ou

Catching Up or Latecomer Advantage? Lessons from e-Research Strategies in Germany, in the UK and Beyond

This paper will discuss the e-Research strategies in Germany, the UK and elsewhere with a view to identifying potential lessons that can be learned. The UK e-Science initiative has been ongoing for more than five years, as has the cyberinfrastructure initiative in the US. The German D-Grid and other e-Research initiatives are much more recent. Generic lessons identified from our cross-national observations include the need to take usability into consideration at the outset of programmes; ‘soft infrastructure’ issues, such as legal and ethical considerations, can delay technological developments; ‘Grid-based infrastructure’ can seem irrelevant to domains outside of large-scale research, complex security systems often lead to ‘work-arounds’ that undermine the integrity of the system; and uptake beyond the initial development group is highly uncertain

Partijwisseling, schorsing en hervatting tijdens het geding. Enkele beschouwingen naar aanleiding van HR 27 mei 2005, NJ 2006, 598, m.nt. HJS.

FDR Procesrecht en mediation -- ou

Modeling Population Growth in R with the biogrowth Package

The growth of populations is of interest in a broad variety of fields, such as epidemiology, economics or biology. Although a large variety of growth models are available in the scientific literature, their application usually requires advanced knowledge of mathematical programming and statistical inference, especially when modelling growth under dynamic environmental conditions. This article presents the biogrowth package for R, which implements functions for modelling the growth of populations. It can predict growth under static or dynamic environments, considering the effect of an arbitrary number of environmental factors. Moreover, it can be used to fit growth models to data gathered under static or dynamic environmental conditions. The package allows the user to fix any model parameter prior to the fit, an approach that can mitigate identifiability issues associated to growth models. The package includes common S3 methods for visualization and statistical analysis (summary of the fit, predictions, . . . ), easing result interpretation. It also includes functions for model comparison/selection. We illustrate the functions in biogrowth using examples from food science and economy

The short-chain fatty acid uptake fluxes by mice on a guar gum supplemented diet associate with amelioration of major biomarkers of the metabolic syndrome

Studies with dietary supplementation of various types of fibers have shown beneficial effects on symptoms of the metabolic syndrome. Short-chain fatty acids (SCFAs), the main products of intestinal bacterial fermentation of dietary fiber, have been suggested to play a key role. Whether the concentration of SCFAs or their metabolism drives these beneficial effects is not yet clear. In this study we investigated the SCFA concentrations and in vivo host uptake fluxes in the absence or presence of the dietary fiber guar gum. C57Bl/6J mice were fed a high-fat diet supplemented with 0%, 5%, 7.5% or 10% of the fiber guar gum. To determine the effect on SCFA metabolism, C-13-labeled acetate, propionate or butyrate were infused into the cecum of mice for 6 h and the isotopic enrichment of cecal SCFAs was measured. The in vivo production, uptake and bacterial interconversion of acetate, propionate and butyrate were calculated by combining the data from the three infusion experiments in a single steady-state isotope model. Guar gum treatment decreased markers of the metabolic syndrome (body weight, adipose weight, triglycerides, glucose and insulin levels and HOMA-IR) in a dose-dependent manner. In addition, hepatic mRNA expression of genes involved in gluconeogenesis and fatty acid synthesis decreased dose-dependently by guar gum treatment. Cecal SCFA concentrations were increased compared to the control group, but no differences were observed between the different guar gum doses. Thus, no significant correlation was found between cecal SCFA concentrations and metabolic markers. In contrast, in vivo SCFA uptake fluxes by the host correlated linearly with metabolic markers. We argue that in vivo SCFA fluxes, and not concentrations, govern the protection from the metabolic syndrome by dietary fibers

Melken in Amerika

Resultaten van een Wagenings afstudeerproject, waarbij een vergelijking werd opgesteld om te bepalen welke bedrijfsontwikkelingsstrategie voor een Nederlandse melkveehouder het meest interessant is: blijven boeren in Nederland of emigreren naar de V.S. en zo ja, naar welk gebied

PIKES Analysis Reveals Response to Degraders and Key Regulatory Mechanisms of the CRL4 Network

Co-opting Cullin4 RING ubiquitin ligases (CRL4s) to inducibly degrade pathogenic proteins is emerging as a promising therapeutic strategy. Despite intense efforts to rationally design degrader molecules that co-opt CRL4s, much about the organization and regulation of these ligases remains elusive. Here, we establish protein interaction kinetics and estimation of stoichiometries (PIKES) analysis, a systematic proteomic profiling platform that integrates cellular engineering, affinity purification, chemical stabilization, and quantitative mass spectrometry to investigate the dynamics of interchangeable multiprotein complexes. Using PIKES, we show that ligase assemblies of Cullin4 with individual substrate receptors differ in abundance by up to 200-fold and that Cand1/2 act as substrate receptor exchange factors. Furthermore, degrader molecules can induce the assembly of their cognate CRL4, and higher expression of the associated substrate receptor enhances degrader potency. Beyond the CRL4 network, we show how PIKES can reveal systems level biochemistry for cellular protein networks important to drug development